The Sekelsky Lab

pP{70wi}

pP{70wi} is a small vector for P element-mediated germline transformation. We have not yet used this vector in transformation. However, two other labs have reported problems obtaining transformants with pP{70w}. We haven't determined what the source of this difficulty is. We have used this hsp70::white cDNA in other constructs, and it works well in our hands. However, we've now inserted an intron back into white, in the hopes that this will increase expression.

pP{70w} Sequence

CATGATGAAATAACATAAGGTGGTCCCGTCGGCAAGAGACATCCACTTAACGTATGCTTG CAATAAGTGCGAGTGAAAGGAATAGTATTCTGAGTGTCGTATTGAGTCTGAGTGAGACAG CGATATGATTGTTGATTAACCCTTAGCATGTCCGTGGGGTTTGAATTAACTCATAATATT AATTAGACGAAATTATTTTTAAAGTTTTATTTTTAATAATTTGCGAGTACGCAAAgctag ctatagttctagaggtaccggttgttaactagtggatccaggcctgaattcgcatgcata tggcggccgcctgcagctggcgccatcgatacgcgtacgtcgcgaccgcggacatgtaca gagctcgagtttttttttttttttttttttttttttattggcacaaatataattacatta gctttttgagggggcaataaacagtaaacacgatggtaataatggtaaaaaaaaaaaaca agcagttatttcggatatatgtcggCTACTCCTTGCGTCGGGCCCGAAGTCTTAGAGCCA GATATGCGAGCACCCGGAAGCTCACGATGAGAATGGCCAGACCCACGTAGTCCAGCGGCA GATCGGCGGCGGAGAAGTTAAGCGTCTCCAGGATGACCTTGCCCGAACTGGGGCACGTGG TGTTCGACGATGTGCAGCTAATTTCGCCCGGCTCCACGTCCGCCCATTGGTTAATCAGCA GACCCTCGTTGGCGTAACGGAACCATGAGAGGTACGACAACCATTTGAGGTATACTGGCA CCGAGCCCGAGTTCAAGAAGAAGCCGCCAAAGAGCAGGAATGGTATGATAACCGGCGGAC CCACAGACAGCGCCATCGAGGTCGAGGAGCTGGCGCAGGATATTAGATATCCGAAGGACG TTGACACATTGGCCACCAGAGTGACCAGCGCCAGGCAGTTGAAGAAGTGCAGCACTCCGG CCCGCAGTCCGATCATCGGATAGGCAATCGCCGTGAAGACCAGTGGCACTGTGAGAAAAA GCGGCAATTCGGCAATCGTTTTGCCCAGAAAGTATGTGTCACAGCGATAAAGTCGACTTC GGGCCTCCCTCATAAAAACTGGCAGCTCTGAGGTGAACACATTTATCGTGGCAAAGACGT TTTGAAAGGTCATGTTGGTCAGGAAGAGGAAGATGGCTCCGTTGATATTCATCACACCCA CTTGCGTGAGTTGTTGGCCCAAAAAGATGAGGCCAATCAAGATGGCAACCATCGTTGTCT GAATAAGTCGCACTTTTACGAGGAGTGGTTCCTTGAGCACCGACAGCCAGGATCGCCACA GGACCGCCCGGAACTGCATGAACCAGGTGGCCTTGTAGGTGTACCCATTCTCCGGCTGCT CCAGTGGCTTCTCCAGATTTTTGGTGGCCAACAACTGCTCCATATCCCGGGCTACTTTGC TAATGGCAAAATTGTCGCATATCTTGGCGATCCGATCACGGGACTCGATCTCCCGTCCGG GCACAACGGCCAACACCTGTACGTAAAAGTCCGCCGGATTGTAGTTGGTAGGACACTGGG CACCCACGctggataggagttgagatgtaatgtaatgctagatacccttaataaacacat cgaactcacTAGGAAAAGAAGTCGACGGCTTCGCTGGGAGTGCCCAAGAAAGCTACCCTG CCCTCGGCCATCAGAAGGATCTTGTCAAAGAGCTCAAACAGCTCGGAAGACGGCTGATGA ATGGTCAGGATGACGGTCTTGCCCTTCTGCGACAGCTTCTTCAGCACCTGGACGACGCTG TGGGCGGTAAATGAGTCCAGTCCGGAGGTGGGCTCATCGCAGATCAGAAGCGGCGGATCG GTTAGTGCCTCGGAGGCGAATGCCAGACGCTTCCTTTCTCCGCCGGACAGACCTTTCACC CTGCCGGGCACACCGATGATCGTGTGCTGACATTTGCTGAGCGAAAGCTCCTGGATCACC TGATCCACGCGGGCCACTCGCTGCCGATAGGTCAGATGTCGTGGCATCCGCACCATGGCT TGGAAAATCAGGTGTTCCCTGGCCGTTAGGGAGCCGATAAAGAGGTCATCCTGCTGGACA TAGGCGCACCTGGCCTGCATCTCCTTGGCGTCCACAGGTTGGCCATTGAGCAGTCGCATC CCGGATGGCGATACTTGGATGCCCTGCGGCGATCGAAAGGCAAGGGCATTCAGCAGGGTC GTCTTTCCGGCACCGGAACTGCCCATCACGGCCAAAAGTTCGCCCGGATAGGCCACGCCG CAAACGTTCTTGAGCAAATGTTTCCTGGGCGCCGGTATGTGTCGCTCGTTGCAGAATAGT CCGCGTGTCCGGTTGACCAGCTGCCGCCATCCGGAGCCCGGCTGATTGACCGCCCCAAAG ATGTCCATATTGTGCCAGGCATAGGTGAGGTTCTCGGCTAGTTGGCCGCTCCCTGAACCG GAGTCCTCCGGCGGACTGGGTGGCAGGAGCGTGCCGTAGTTTTTGGCCTGCCCGAAGCCC TGGTTAATGCAGCTCTGCGAAGCCGCTCCGCTGTCACCATTGTTCAGATGCTCGGCAGAT GGGTGTTTGCTGCCTCCGCGAATTAATAGCTCCTGATCCTCTTGGCCCATtgccgggatt tttcacactttcccctgcttacccacccaaaaccaatcacccacccaatcactcaaaaaa caaacaaaaataagaagcgagaggagttttggcacagcactttgtgtttaattgatggcg taaaccgcttggagcttcgtcacgaaacgcctcgtgccgaattgacgctatcgacctatt cAGAGTTCTCTTCTTGTCTTCAATAATGACTTCTTGGTTGATTTCAGTAGTTGCAGTTTT TAGTTTAATTACTTGGTTGTTGGTTACTTTTAATTGATTCACTTTAACTTGCACTTTATT GCAGATTGTTTAGCTTGTTCAGCTGCGCTTGTTTGTTTGCTCAGCTTACGCTTCGCGATG TGTTCACTTTGcttgtttgaattgaattgacgctccgtcgacgaagcgcctctatttata ctccggcgctcttttcgcgaacattcgaggcgcgctctctcgaagcaacgagaatagcgt gccgtttactgtgcgacagagtgagagagcaatagtacagagagggagagtcacaaaacg aatagagaataacggggggatccactagcggctgctgctctAAACGACGCATTTCGTACT CCAAAGTACGAATTTTTTCCCTCAAGCTCTTATTTTCATTAAACAATGAACAGGACCTAA CGCACAGTCACGTTATTGTTTACATAAATGATTTTTTTTACTATTCAAACTTACTCTGTT TGTGTACTCCCACTGGTATAGCCTTCTTTTATCTTTTCTGGTTCAGGCTCTATCACTTTA CTAGGTACGGCATCTGCGTTGAGTCGCCTCCTTTTAAATGTCTGACCTTTTGCAGGTGCA GCCTTCCACTGCGAATCATTAAAGTGGGTATCACAAATTTGGGAGTTTTCACCAAGGCTG CACCCAAGGCTCTGCTCCCACAATTTTCTCTTAATAGCACACTTCGGCACGTGAATTAAT TTTACTCCAGTCACAGCTTTGCAGCAAAATTTGCAATATTTCATTTTTTTTTATTCCACG TAAGGGTTAATGTTTTCAAAAAAAAATTCGTCCGCACACAACCTTTCCTCTCAACAAGCA AACGTGCACTGAATTTAAGTGTATACTTCGGTAAGCTTCGGCTTTCGACGGGACCACCTT ATGTTATTTCATCATGggccagacccacgtagtccagcggcagatcggcggcggagaagt taagcgtctccaggatgaccttgcccgaactggggcacgtggtgttcgacgatgtgcagc taatttcgcccggctccacgtccgcccattggttaatcagcagaccctcgttggcgtaac ggaaccatgagaggtacgacaaccatttgaggtatactggcaccgagcccgagttcaaga agaaggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaa gtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagct ccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcc cttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtagg tcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgcct tatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcag cagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttga agtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctga agccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctg gtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaag aagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaag ggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaat gaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgct taatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgac tccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaa tgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccg gaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaatt gttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgcca ttgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggtt cccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctcct tcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatgg cagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtg agtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccgg cgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaa aacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgt aacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggt gagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgtt gaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctca tgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacat ttccccgaaaagtgccacctgcgaagtcttagagccagatatgcgagcacccggaagctc
acgatgagaatggccagac

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